Animal tests ―More than a cosmetic (化妆品) change Every time you reach for an eye-drop or reapply a lip salve, you do be so confident that the chemicals they contain are safe to use. But the toxicology (毒物学) tests on which regulators rely to gather this information are largely based on wasteful and often poorly predictive animal experiments. Efforts in Europe are about to change this, and the man charged with bringing toxicology into the twenty-first century is a plain-talking German: Thomas Hartung. Although Hartung acknowledges the immense challenges ahead, he sees this as an opportunity for toxicology "to turn itself at last into a respectable science". Three years ago, when Hartung became director of the European Centre for the Validation of Alternative Methods (ECVAM) in Ispra, Italy. ECVAM was set up in 1993 to support European Union policy aimed at reducing the number of animals used in regulatory testing. The centre, which sits on the sleepy shores of Lake Maggiore in the Italian Alps, originally had ten members of staff and faced an uphill struggle to cut back the millions of animal tests carried out in Europe every year. Then in 2003, two major policy changes were announced from above, increasing the pressure on the centre’’s labs. ECVAM found itself facing an unexpectedly short deadline for delivering a slew of animal-free methods for testing chemical toxicity.Rule change The first change was to the European Union’’s Cosmetics Directive, which phases out over ten years the use of animals in cosmetics testing. A short while later, the European Commission proposed its controversial REACH legislation (Registration, Evaluation and Authorization of Chemicals). Europe produces some 30,000 chemicals for which toxicity data have never been registered. REACH aims to make registration mandatory for both future and existing chemicals ― even those that have been on the market for decades. If, as expected, the REACH directive is approved next year, it will come into effect in 2007. Animal-welfare groups fear that this will mean millions more animals will be used in tests to meet the regulatory requirements. And industry claims that the testing process could cost billions of euros. Almost overnight, industry’’s interest in cheaper, animal-free testing skyrocketed. Last month ECVAM was put in charge of developing, with industry and regulatory agencies, the testing strategies for REACH. Now commanding 50 staff, Hartung is rising to the challenge. "The toxicity tests that have been used for decades are simply bad science", he explains. "We now have an opportunity to start with a clean slate and develop evidence-based tests that have true predictive value." Many of the animal tests used today were developed under crisis conditions. The notorious Draize test, which assesses the irritation or damage caused by chemicals simply by putting them into the eyes of rabbits, is a prime example. It was developed by the US Food and Drug Administration in 1944 after reports in the 1930s that some cosmetics were causing permanent eye injuries. One 38-year-old woman had gone blind after dyeing her lashes with Lash-Lure, a product that contained a derivative of coal tar. Then came the calamity of thalidomide (镇静剂), which was given to pregnant women in the late 1950s to control morning sickness, but which caused horrific birth defects. By this time, governments were highly sensitive to public concerns and called on their authorities to develop animal-based tests that would predict all conceivable toxic effects of drugs and chemicals. The principles behind most of those tests remain more or less unchanged today.Safety catch Each chemical that goes through the multiple tests required for registration can use up to 5,000 animals ― or 12,000 if the chemical is a pesticide. The cost of doing this for the 30,000 unregistered chemicals so that they comply with REACH has been estimated at between 5 billion (US6 billion) and 10 billion. In the decade since ECVAM was established, the number of animals used in toxicology testing has fallen slightly, although it still hovers at about one million per year. This reduction is a result of the refinement of existing tests, and the introduction of some alternative methods that rely on in vitro (试管) tests using cell cultures. ECVAM believes that it can halve the total number of animals used for regulatory testing within a decade. It has just completed its first large-scale validation study of an in vitro cytotoxicity test, which monitors death of cultured cells following short-term exposure to a chemical. Chemicals shown to be harmful in this test would be excluded from any LD50 animal tests. At least 70% of the chemicals registered in the past two decades fall into this category, says Hartung. And this is just the beginning.Poor prediction Most animal tests over-or under-estimate toxicity, or simply don’’t mirror toxicity in humans very well. The relevant industries also acknowledges the poor quality of those tests. Take the embryotoxicity test in which chemicals are fed to pregnant animals and the fates of their embryos, and the progeny of two subsequent generations, are studied. "Animal embryotoxicity tests are not reliably predictive for humans," says Horst Spielmann, a toxicologist at the Federal Institute for Risk Assessment in Berlin. "When we find that cortisone is embryotoxic in all species tested except human, what are we supposed to make of them " The same goes for cancer. To test a single chemical for its potential to cause cancer takes five years and involves 400 rats, each of which is treated with the maximum tolerated dose. It is dramatically over-predictive: more than 50% of the results are positive, of which 90% are false positive. Yet the number of compounds proved to be carcinogenic to humans is very low ― the International Agency for Research on Cancer in Lyons, France, has identified just 95 proven and 66 probable human carcinogens (致癌物质).Life or death Scientists also cannot assume that in vitro alternatives are automatically better, says Spielmann. In 1971, a comparison of animal Draize tests in different labs revealed the test to be hopelessly non-reproducible. But Spielmann’’s 1995 study of animal-free alternatives to the Draize test showed that they were equally unreliable. Since then the in vitro tests have been standardized, and they are intrinsically more reproducible. "Although reproducibility and relevance are not the same thing," Spielmann cautions. Relevance requires a good match between the test results and human data. At an ECVAM workshop in February, 30 industrial scientists met to develop the most effective strategy for using the alternative Draize tests, so that the false negatives and false positives of each test compensate for each other. This strategy is now going through the crucial validation procedure, in which human data, often from occupational health databases, will be used as points of reference. ECVAM has so far seen 17 alternative tests through validation ― 11 use in vitro methods, another six involve refining in vivo tests to reduce the number of animals used. Most of the new tests assess acute toxicity, but animal use is highest when testing for the toxic effects of prolonged exposure to chemicals for long-term consequences such as cancer and reproductive toxicity. These costly procedures are harder to mimic in vitro and may never be completely replaced.Sounds familial This is why, apart from the 30 million it uses to support ECVAM annually, the European Commission is funding three multimillion-euro ’’Integrated Projects’’. Under these, dozens of labs will collaborate for five years to tackle more difficult issues, such as allergic reactions (过敏反应) or widespread toxicity resulting from chemicals entering the bloodstream. Scientists know that they are likely to find it hardest to convince regulators about alternative tests for highly emotive issues such as cancer and birth defects. More than half of all animals that will be needed to support REACH legislation are likely to be used in reproductive toxicology testing. The 9-million Integrated Project called ReProTect has 27 labs dedicated to developing alternatives to these tests. The ReProTect consortium has broken down the human reproductive cycle into smaller elements, from male and female fertility to implantation, to pre-and postnatal development, and is trying to develop a meaningful package of tests. "Quite correctly everyone feels uneasy about taking risks where stakes are so high and issues so emotive," says Hartung. "We all want to be sure that there is real evidence that alternative tests are predictive of human toxicity." For example, regulators know the weaknesses of the rat cancer test as well as scientists but, wanting to be safe rather than sorry, they accept it because it is believed to throw up few false negatives. They prefer to let industry prove the innocence of any compound that shows up positive. Any replacement tests will need to reassure both regulators and industry.How is an embryotoxicity test carried out

Animal tests ―More than a cosmetic (化妆品) change Every time you reach for an eye-drop or reapply a lip salve, you do be so confident that the chemicals they contain are safe to use. But the toxicology (毒物学) tests on which regulators rely to gather this information are largely based on wasteful and often poorly predictive animal experiments. Efforts in Europe are about to change this, and the man charged with bringing toxicology into the twenty-first century is a plain-talking German: Thomas Hartung. Although Hartung acknowledges the immense challenges ahead, he sees this as an opportunity for toxicology "to turn itself at last into a respectable science". Three years ago, when Hartung became director of the European Centre for the Validation of Alternative Methods (ECVAM) in Ispra, Italy. ECVAM was set up in 1993 to support European Union policy aimed at reducing the number of animals used in regulatory testing. The centre, which sits on the sleepy shores of Lake Maggiore in the Italian Alps, originally had ten members of staff and faced an uphill struggle to cut back the millions of animal tests carried out in Europe every year. Then in 2003, two major policy changes were announced from above, increasing the pressure on the centre’’s labs. ECVAM found itself facing an unexpectedly short deadline for delivering a slew of animal-free methods for testing chemical toxicity.Rule change The first change was to the European Union’’s Cosmetics Directive, which phases out over ten years the use of animals in cosmetics testing. A short while later, the European Commission proposed its controversial REACH legislation (Registration, Evaluation and Authorization of Chemicals). Europe produces some 30,000 chemicals for which toxicity data have never been registered. REACH aims to make registration mandatory for both future and existing chemicals ― even those that have been on the market for decades. If, as expected, the REACH directive is approved next year, it will come into effect in 2007. Animal-welfare groups fear that this will mean millions more animals will be used in tests to meet the regulatory requirements. And industry claims that the testing process could cost billions of euros. Almost overnight, industry’’s interest in cheaper, animal-free testing skyrocketed. Last month ECVAM was put in charge of developing, with industry and regulatory agencies, the testing strategies for REACH. Now commanding 50 staff, Hartung is rising to the challenge. "The toxicity tests that have been used for decades are simply bad science", he explains. "We now have an opportunity to start with a clean slate and develop evidence-based tests that have true predictive value." Many of the animal tests used today were developed under crisis conditions. The notorious Draize test, which assesses the irritation or damage caused by chemicals simply by putting them into the eyes of rabbits, is a prime example. It was developed by the US Food and Drug Administration in 1944 after reports in the 1930s that some cosmetics were causing permanent eye injuries. One 38-year-old woman had gone blind after dyeing her lashes with Lash-Lure, a product that contained a derivative of coal tar. Then came the calamity of thalidomide (镇静剂), which was given to pregnant women in the late 1950s to control morning sickness, but which caused horrific birth defects. By this time, governments were highly sensitive to public concerns and called on their authorities to develop animal-based tests that would predict all conceivable toxic effects of drugs and chemicals. The principles behind most of those tests remain more or less unchanged today.Safety catch Each chemical that goes through the multiple tests required for registration can use up to 5,000 animals ― or 12,000 if the chemical is a pesticide. The cost of doing this for the 30,000 unregistered chemicals so that they comply with REACH has been estimated at between 5 billion (US$6 billion) and 10 billion. In the decade since ECVAM was established, the number of animals used in toxicology testing has fallen slightly, although it still hovers at about one million per year. This reduction is a result of the refinement of existing tests, and the introduction of some alternative methods that rely on in vitro (试管) tests using cell cultures. ECVAM believes that it can halve the total number of animals used for regulatory testing within a decade. It has just completed its first large-scale validation study of an in vitro cytotoxicity test, which monitors death of cultured cells following short-term exposure to a chemical. Chemicals shown to be harmful in this test would be excluded from any LD50 animal tests. At least 70% of the chemicals registered in the past two decades fall into this category, says Hartung. And this is just the beginning.Poor prediction Most animal tests over-or under-estimate toxicity, or simply don’’t mirror toxicity in humans very well. The relevant industries also acknowledges the poor quality of those tests. Take the embryotoxicity test in which chemicals are fed to pregnant animals and the fates of their embryos, and the progeny of two subsequent generations, are studied. "Animal embryotoxicity tests are not reliably predictive for humans," says Horst Spielmann, a toxicologist at the Federal Institute for Risk Assessment in Berlin. "When we find that cortisone is embryotoxic in all species tested except human, what are we supposed to make of them " The same goes for cancer. To test a single chemical for its potential to cause cancer takes five years and involves 400 rats, each of which is treated with the maximum tolerated dose. It is dramatically over-predictive: more than 50% of the results are positive, of which 90% are false positive. Yet the number of compounds proved to be carcinogenic to humans is very low ― the International Agency for Research on Cancer in Lyons, France, has identified just 95 proven and 66 probable human carcinogens (致癌物质).Life or death Scientists also cannot assume that in vitro alternatives are automatically better, says Spielmann. In 1971, a comparison of animal Draize tests in different labs revealed the test to be hopelessly non-reproducible. But Spielmann’’s 1995 study of animal-free alternatives to the Draize test showed that they were equally unreliable. Since then the in vitro tests have been standardized, and they are intrinsically more reproducible. "Although reproducibility and relevance are not the same thing," Spielmann cautions. Relevance requires a good match between the test results and human data. At an ECVAM workshop in February, 30 industrial scientists met to develop the most effective strategy for using the alternative Draize tests, so that the false negatives and false positives of each test compensate for each other. This strategy is now going through the crucial validation procedure, in which human data, often from occupational health databases, will be used as points of reference. ECVAM has so far seen 17 alternative tests through validation ― 11 use in vitro methods, another six involve refining in vivo tests to reduce the number of animals used. Most of the new tests assess acute toxicity, but animal use is highest when testing for the toxic effects of prolonged exposure to chemicals for long-term consequences such as cancer and reproductive toxicity. These costly procedures are harder to mimic in vitro and may never be completely replaced.Sounds familial This is why, apart from the 30 million it uses to support ECVAM annually, the European Commission is funding three multimillion-euro ’’Integrated Projects’’. Under these, dozens of labs will collaborate for five years to tackle more difficult issues, such as allergic reactions (过敏反应) or widespread toxicity resulting from chemicals entering the bloodstream. Scientists know that they are likely to find it hardest to convince regulators about alternative tests for highly emotive issues such as cancer and birth defects. More than half of all animals that will be needed to support REACH legislation are likely to be used in reproductive toxicology testing. The 9-million Integrated Project called ReProTect has 27 labs dedicated to developing alternatives to these tests. The ReProTect consortium has broken down the human reproductive cycle into smaller elements, from male and female fertility to implantation, to pre-and postnatal development, and is trying to develop a meaningful package of tests. "Quite correctly everyone feels uneasy about taking risks where stakes are so high and issues so emotive," says Hartung. "We all want to be sure that there is real evidence that alternative tests are predictive of human toxicity." For example, regulators know the weaknesses of the rat cancer test as well as scientists but, wanting to be safe rather than sorry, they accept it because it is believed to throw up few false negatives. They prefer to let industry prove the innocence of any compound that shows up positive. Any replacement tests will need to reassure both regulators and industry.How is an embryotoxicity test carried out